The resistance in the coils converts the electrical energy into heat energy which causes the coils to release heat. An inoculating needle is used for inoculating an agar deep. This device creates a sterile environment with the flow of sterile air through a High-Efficiency Particulate Air (HEPA) filter and shortwave ultraviolet germicidal lamp that sterilizes the workstation. Contextual animations in each section give users good illustrations of technique details and related theories. A hot air oven is an electrical device that is used for sterilization of medical equipment or samples using dry heat. The instruments used in the microbiology labs include a bunch of different kinds of instruments required for a lot of different processes conducted within those laboratories. Figure: Instruments used in Microbiology Lab with Principle and Uses. 1. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. This will prevent any foreign contaminants from coming in contact with the customers and sample during testing. Other than that microbiology is also an applied science that assists agriculture, environmental sector, health and medicine and the growing biotechnology industry. In microbiology lab, it is used for maintaining pH of the medium and diluents. The field is concerned with the structure, function, and classification of such organisms and with ways of … The transmission of infectious diseases via contaminated water continues to be a risk to public health in the United States and throughout the rest of the. Source and finished drinking waters are vulnerable to microbial pathogen contamination from a variety of sources of human and animal fecal wastes and from the introduction and proliferation of nonfecal pathogenic microbes. Additionally, techniques such as immunofluorescence and fluorescence in situ hybridization (FISH), in which fluorescent probes are used to bind to DNA, can be used. A pH meter is often termed as “potentiometric pH meter” as it measures the difference in electric potential between the reference and a pH electrode. Image created with biorender.com. It is coupled with a heating system to heat the liquid while it mixed. Static air hot air oven: In this type of oven, the heat is produced by coils present at the bottom of the oven with no fan. Spread plate method is used for culture of special clonal colonies and pour plate method is used for counting the specific number of colonies in a liquid medium. The primary application of a centrifuge is the separation of particles suspended in a suspension. classification of culture media used in microbiology laboratory on the basis of consistency Solid medium: – Solid medium contains agar at a concentration of 1.5-2.0% or some other, mostly inert solidifying agent. While perhaps best known to us as a cause of human disease, bacteria really should be far more famous for their positive contributions than for their negative ones. Designed with ❤️ by Sagar Aryal. Sometime it is needed to suppress the growth of some particular bacteria, in that case some measured amount of concentrates are added to the water and they are called selective media. In a laboratory tabletop centrifuge, the sample tubes are aligned at an angle so that the particles have to travel a shorter distance before they hit the bottom. Heat fixed smears are ready for staining. Water Bath is a conventional device that is used for chemical reactions that required a controlled environment at a constant temperature. It is commonly used for processes like sterilization, combustion, and heating. The agar deep medium is stabbed, not streaked. Disease-causing bacteria usually don’t visible to naked eye and hence a variety of sterilization techniques are employed in microbiology. An autoclave is a pressurized chamber used for the process of sterilization and disinfection by combining three factors: time, pressure and steam. These are suitable for heating chemicals that might be flammable under direct ignition. In medical or microbiology laboratories, it is commonly used for micro-loop sterilization. Negative stains are used when a specimen or a part of it, such as the capsule resists taking up the stain. It is used to obtain distilled water required for many lab tests as well as for the preparation of culture media. Incubators have a heating system that allows for the temperature within the incubator to be adjusted according to the type of organism cultivated inside. 1st Year General Microbiology Syllabus (Nepal), T.U. A centrifuge is a device that allows the rotation of an object about a single axis, where an outward force is applied perpendicularly to the axis. This device is used in place of other stirrers as it is noise-free and because the size of the stir bar is so tiny, there is less chance of contamination. An inoculating loop is used for transferring the microorganism. In a simple stain, dyes that are either attracted by charge (a cationic dye such as methylene blue or crystal violet) or repelled by charge (an anionic dye such as eosin or India ink) are added to the smear. For this effective sterilization techniques are designed and studied in microbiology. Depending on the growth requirements of the bacteria, mineral and vitamins may also be provided. Home » Basic Microbiology » Instruments used in Microbiology Lab with Principle and Uses, Last Updated on June 17, 2020 by Sagar Aryal. To Aid in … Laboratory techniques in microbiology. Centrifugation 4. The equipment inside the oven acquire heat and pass the heat towards the center, one layer at a time which allows for effective dry heat sterilization. Similarly, they are provided with adjustments for maintaining the concentration of CO2 to balance the pH and humidity required for the growth of the organisms. Tagging can be done in various ways, such as nick translation, or PCR using tagged nucleotides. The instruments used in the microbiology labs include a bunch of different kinds of instruments required for a lot of different processes conducted within those laboratories. for a more extended period of time. Instruments used specially in microbiology include: Instrument list. Variation of the incubator like a shaking incubator is also available, which allows for the continuous movement of the culture required for cell aeration and solubility studies. Microbes who can tolerate the constituents of the selective media can only grow in that selective media. Like some are responsible for so many potential diseases in human and other living beings while others are utilized for benefit of our environment. _____________… ______________________________________________________________________ 3. pH meter is an electrical instrument used for measuring hydrogen ion concentration of solutions and mixtures (Fig. CO1: Describe construction and Working of common instruments used in Microbiology laboratory CO2: Explain use of various glassware used in microbiology experiments CO3: Handle and use compound microscope to observe microorganisms CO4: Prepare and stain the smear and focus the slide to observe bacterial/ fungal specimens or Examples of light microscopes include brightfield microscopes, darkfield microscopes, phase-contrast microscopes, differential interference contrast microscopes, fluorescence microscopes, confocal scanning laser microscopes, and two-photon microscopes. Organic chemistry. An analytical balance is a type of balance that is commonly used for the measurement of mass in the sub-milligram range. The most common technique is by spreading on the surface of the agar or in the broth with the help of a heat sterilized loop. There are two types of hot air oven based on the working principle. Many types of microscopes fall under the category of light microscopes, which use light to visualize images. Hence in the identification of bacteria, we use microscopical, medical, biochemical, & serological methods. Incubators have a wide range of applications including cell culture, pharmaceutical studies, hematological studies, and biochemical studies. That’s your senses coming alive as warmer, brighter days. They are primarily used for the observation of minute particles which cannot be observed with naked eyes. This site uses Akismet to reduce spam. Different wavelengths of lights are passed through a solution as different substances have better absorbance at different wavelengths. It is based on the simple principle of causing reactions and homogenization by agitating the mixture. The hot air rises and doesn’t allow the effective sterilization of the materials. Save my name, email, and website in this browser for the next time I comment. The loop is a piece of wire that is looped at one end. Acinetobacter baumannii- Lab Diagnosis, Treatment and Prevention, E. coli- Lab Diagnosis, Treatment, Prevention and Control, Staphylococcus aureus- Lab Diagnosis, Treatment, Prevention, Klebsiella pneumoniae- Lab Diagnosis, Treatment, Prevention, Microbiology of extreme environments (Types and Examples), General Aseptic Techniques in Microbiology Laboratory, Top 10 Microbiology Universities in Germany (Updated 2020), Top 10 Microbiology Journals with Impact Factor (updated 2019), T.U. 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